TRAIL is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells.
Using a combination of PCR and library screening, the authors isolated cDNAs corresponding to the TRAILR2 coding region.
The predicted 440aa protein contains a putative signal peptide, 2 cysteine-rich pseudorepeats characteristic of TNF receptor (TNFR) family members, a transmembrane domain, and an intracellular death domain.
The death domain of TRAILR2 shares 30% identity with that of TNFR1.
Overall, the TRAIL receptors TRAILR2 and TRAILR1 (DR4) are 58% identical.
Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TNFRSF10B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser234-Ala435 of human TNFRSF10B, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
