TNF-beta is a potent mediator of inflammatory and immune responses.TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha.
Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission.
TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface.
TNF-beta is cytotoxic to a wide range of tumor cells.
LTA is highly inducible, secreted, and exists as homotrimeric molecule.
LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TNFb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ser35-Ser191 of rat TNFb, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
