TSG-6 is a 30kD secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins.
The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration.
This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IlphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation.
The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1).
The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TNFaIP6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Trp18-Leu275 of mouse TNFaIP6, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
