84-4439-67　TNFa (Tumor Necrosis Factor Alpha, DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2) 200ul　365351

特徴

• TNFa  is synthesized as a 26kD, type II transmembrane protein that is 233aa in length.
• It contains a 30aa cytoplasmic domain, a 26aa transmembrane segment, and a 177aa extracellular region.
• TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein.
• The 157aa residue soluble form of TNFa (sTNF-alphais released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase.
• Rat cells known to express TNF-alphainclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LRS SSRTPSDKPV AHVVANPQAE GQLQWLSGRA NALLANGVKL TDNQLVVPLD GLYLIYSQVL FKGQGCPSTH VLLTHTISRL AVSYPSKVNL LSAIKSPCHT ESPEQAEAKP WYEPIYLGGV FQLEKGDQLS AEINQPNYLD FAESGQVYFG IIAL
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.