TNFa is synthesized as a 26kD, type II transmembrane protein that is 233aa in length.
It contains a 30aa cytoplasmic domain, a 26aa transmembrane segment, and a 177aa extracellular region.
TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein.
The 157aa residue soluble form of TNFa (sTNF-alphais released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase.
Rat cells known to express TNF-alphainclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes chicken TNFa.
Isotype：IgY
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ch
Immunogen：Recombinant protein corresponding to Arg1-Phe73 of chicken TNFa, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
