84-4439-64　TNC (Tenascin C, TN-C, HXB, TN, GMEM, JI, Myotendinous antigen, Neuronectin, GP 150-225, Cytotactin, Hexabrachion, Glioma-associated-extracellular matrix antigen) 200ul　365348

特徴

• Tenascinis an extracellular matrix protein with a spatially and temporally restricted tissue distribution.
• It is a hexomeric, multidomain protein with disulfide-linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia, in tendon anlagen, and in developing cartilage and bone.
• In the adult tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle.
• Pearson et al.
• (1988) isolated cDNA clones coding for tenascin from a chicken fibroblast cDNA expression library using a specific tenascin antiserum.
• They showed induction of tenascin in vitro by fetal calf serum as well as by transforming growth factor-beta .
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CVCEPGW KGPNCSEPDC PGNCNLRGQC LDGQCICDEG FTGEDCSQLA CPNDCNDQGR CVNGVCVCFE GYAGPDCGLE VCPVPCSEEH GMCVDGRCVC KDGFAGEDCN EPLCLNNCYN RGRCVENECV CDEGFTGEDC SELICPNDCF DRGRCINGTC YCEEGFTGED CGELTCPNDC QGRGQCEEGQ CVCNEGFAGA DCSEKRCPAD CHHRGRCLNG QCECDDGFTG ADCGDLQCPN GCSGHGRCVN GQCVCDEGYT GEDCSQRRCP NDCHNRGLCV QGKCICEQGF KGFDCSEMSC PNDCHQHGRC VNGMCICDDD YTGEDCRDRR CPRDCSQRGR CVDGQCICED GFTGPDCAEL SCPSDCHGHG RCVNGQCICH EGFTGKDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG QRSCPNDCSN QGQCVSGRCI CNEGYTGIDC S
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.