TMEM27 encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules.
It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells.
TMEM27 has an N-terminal signal sequence, followed by a noncatalytic extracellular domain, a transmembrane segment, and a cytosolic domain.
The extracellular domain contains an O-glycosylation site and 2 N-glycosylation sites.
Human TMEM27 shares 47.8% identity with the C-terminal end of ACE2, but it lacks an ACE2-like catalytic domain.
Northern blot analysis of rat, mouse, and human tissues detected a transcript of about 1.8 kb exclusively in kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TMEM27.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile12-Leu222 of mouse TMEM27, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
