Toll-like receptors (TLRs) recognize microbial products and initiate a complex immune response designed to eliminate invading pathogens.
TLR9 is specialized to recognize microbial nucleic acids, and 1 factor enabling TLR9 to discriminate between self and foreign DNA is the presence of unmethylated CpG dinucleotides in bacterial DNA (Hasan et al., 2007).
Other TLR proteins, TLR9 contains extracellular leucine-rich repeats (LRRs) and a cytoplasmic toll/interleukin-1R (TIR) domain.
It also has a signal peptide (residues 1 to 25) and a transmembrane domain (residues 819 to 836).
Northern blot analysis of mouse tissues revealed highest Tlr9 expression in spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TLR9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr28-Ser362 of human TLR9, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
