TALIN is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contactand, in lymphocytes, at cell-cell contacts.
Talin-1 is a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts.
This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts.
It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells.
The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions.
The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5,000 Western Blot: 1:50-400 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TLN.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala975-Ser1181 of mouse TLN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
