Transketolase (TKT) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway.
As part of a systematic search for differentially expressed genes, Coy et al.
(1996) isolated a novel transketolase-related gene, TKTL1, which they called TKR.
They isolated transcripts encoding tissue-specific protein isoforms.
Comparison with known transketolases demonstrated a TKR-specific deletion mutating 1 thiamine-binding site
Genomic sequencing of the TKR gene by Coy et al.
(1996) revealed the presence of a pseudoexon, as well as the acquisition of a tissue-specific spliced exon, compared to the TKT gene, which maps to 3p14.3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TKTL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Asp259 of human TKTL1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
