Tight junction protein ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions.
The encoded protein may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified for this gene.
Tight junction (zonula occludens) protein-1 (TJP1), also referred to as ZO-1, is a 200kD protein located on a cytoplasmic membrane surface of vertebrate intercellular tight junctions.
Willott et al.
(1993) isolated a full-length cDNA sequence for human TJP1.
Although the function of TJP1 is unknown, the cDNA sequence predicted a multi-domain signaling protein homologous to the product of the 'discs large-1' tumor suppressor gene of Drosophila and several other membrane-associated proteins in mammals.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TJP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly23-Asp502 of rat TJP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
