characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor- encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al.
Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas.
In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines.
Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis.
Thus, Tachibana et al.
concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TIEG1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Pro206-Pro473 of mouse TIEG1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
