84-4439-21　TIEG1 (TGF Beta Inducible Early Response Gene 1, KLF10, EGRA, TIEG, Krueppel-Like Factor 10, TGFB Inducible Early Growth Response, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1) 200ul　365305

特徴

• Tachibana et al.
• characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor- encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al.
• Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas.
• In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines.
• Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis.
• Thus, Tachibana et al.
• concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MLNFGASLQQ TAEERMEMIS ERPKESMYSW NKTAEKSDFE AVEALMSMSC SWKSDFKKYV ENRPVTPVSD LSEEENLLPG TPDFHTIPAF CLTPPYSPSD FEPSQVSNLM APAPSTVHFK SLSDTAKPHI AAPFKEEEKS PVSAPKLPKA QATSVIRHTA DAQLCNHQTC PMKAASILNY QNNSFRRRTH LNVEAARKNI PCAAVSPNRS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.