By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al.
(1999) cloned THSD7A, which they designated KIAA0960.
The deduced 1290aa protein shares characteristics with proteins involved in cell signaling.
RT-PCR ELISA detected low to moderate expression in all tissues and specific brain regions examined except skeletal muscle, where expression was absent.
By radiation hybrid analysis, Nagase et al.
(1999) mapped the THSD7A gene to chromosome 7.
The thrombospondins (TSP) are a family of multifunctional proteins.
The family consists of thrombospondins 1-5 and can be divided into 2 subgroups: A, which contains TSP-1 and -2, and B, which contains TSP-3, -4 and -5.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2g/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes mouse THSD7A.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Thr42-Tyr242 of mouse THSD7A, expressed in E. coli.
