Transforming growth factor beta 3 (TGF-Beta3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development.
It belongs to a large family of cytokines called the Transforming growth factor beta superfamily, which includes the TGF-Beta family, Bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), inhibins and activins.
TGFBeta3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development.
Without TGF-Beta3, mammals develop a deformity known as a cleft palate.
This is caused by failure of epithelial cells in both sides of the developing palate to fuse.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control (Supplied):365276A: Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes human TGFb3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu24-Arg300 of human TGFb3, expressed in E. coli.
