84-4438-85　TGFb1 (Transforming Growth Factor Beta 1, TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide) 200ul　365271

特徴

• TGF-Beta is capable of producing a variety of effects and virtually all cell types respond to this factor in some way.
• The inappropriate presence of active TGF-Beta1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-Beta is secreted by cells in the form of an inactive complex.
• This complex consists of TGF-Beta1 associated non-covalently with a protein designated the latency associated peptide (LAP).
• TGF-Beta1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion.
• LAP and TGF-Beta1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-Beta1 inactive and inaccessible to anti-TGF-Beta antibodies.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-AL DTNYCFSSTE KNCCVRQLYI DFRKDLGWKW IHEPKGYHAN FCLGPCPYIW SLDTQYSKVL ALYNQHNPGA SAAPCCVPQA LEPLPIVYYV GRKPKVEQLS NMIVRSCKCS
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.