84-4438-76　TFPI (Tissue Factor Pathway Inhibitor, TFI, EPI, LACI, TFPI1, Lipoprotein-Associated Coagulation Inhibitor, Extrinsic pathway inhibitor) 200ul　365262

特徴

• Tissue Factor Pathway Inhibitor (TFPI) circulates in plasma as a complex with LDL>HDL>>VLDV.
• In plasma, TFPI is found in several forms: one at 36,000kD, one at 43,000kD, and the various truncated forms.
• This size heterogeneity appears in part to be the result of the formation of mixed disulfide complexes between TFPI and apolipoprotein AII.
• Approximately 10% of total TFPI is carried by platelets, which release TFPI once they are activated by thrombin.
• Thus, at the site of a wound, where platelets aggregate, elevated levels of TFPI are present.
• TFPI has dual inhibitory function (against factor Xa and factor VIIa/TF).
• This is consistent with the presence of multiple Kunitz-type domains: (Kunitz for factor VIIa/TF and Kunitz for factor Xa interaction).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- DS EEDEEHTIIT DTELPPLKLM HSFCAFKADD GPCKAIMKRF FFNIFTRQCE EFIYGGCEGN QNRFESLEEC KKMCTRDNAN RIIKTTLQQE KPDFCFLEED PGICRGYITR YFYNNQTKQC ERFKYGGCLG NMNNFETLEE CKNICEDGPN GFQVDNYGTQ LNAVNNSLTP QSTKVPSLFV TKEGTNDGWK NAAHIYQVFL NAFCIHASMF FLGLDSISCL C
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap