Trefoil factor 3 is a protein encoded by the TFF3 gene.
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40aa domain that contains three conserved disulfides.
They are stable secretory proteins expressed in gastrointestinal mucosa.
Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.
This gene is expressed in goblet cells of the intestines and colon.
This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes mouse TFF3.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Mo
Immunogen:Recombinant protein corresponding to Ala23-Phe81of mouse TFF3, fused to two N-terminal Tags, His-Tag and MBP-tag, expressed in E. coli.