Trefoil factor 1 is a protein that is encoded by the TFF1 gene.
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40aa domain that contains three conserved disulfides.
They are stable secretory proteins expressed in gastrointestinal mucosa.
Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium
Trefoil factor 1, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors.
This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TFF1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Phe81of rat TFF1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
