The E2F transcription factor family regulates the expression of various cellular promoters, particularly those involved in the cell cycle.
E2F factors bind to DNA as homodimers or heterodimers in association with dimerization partner DP1.
TFDP1 may be the first example of a family of related transcription factors.
The protein was isolated by virtue of its binding to a DNA sequence taken from the adenovirus E2A promoter.
TFDP1 is a component of the DRTF1/E2F transcription factor complex, which coordinates events in the cell cycle by interacting with RB1, the retinoblastoma gene product, and RB-related proteins.
DRTF1 activity is downregulated in differentiating embryonal carcinoma cells, and E2F activity is induced in adenovirus-infected HeLa cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TFDP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly6-Asp410 of human TFDP1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
