Dual specificity testis-specific protein kinase 1 is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain.
Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs)
The encoded protein can phosphorylate myelin basic protein and histone in vitro.
The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis.Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes mouse TESK1.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Mo
Immunogen:Recombinant protein corresponding to Phe52-Ile309 of mouse TESK1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.