Telomerase-associated protein 1 (TEP1) is a component of the telomerase ribonucleoprotein complex, which catalyzes the addition of new telomeres on the chromosome ends.
This activity is mediated by the catalytic telomerase reverse transcriptase (TERT) and telomerase RNA component template (TERC).
The ribonucleoprotein complex responsible for telomerase activity had been purified only in ciliates.
Purified tetrahymena telomerase contains an RNA and 2 protein components, p80 and p95.
The p80 component can be specifically cross linked to telomerase RNA, whereas the p95 component binds and cross links to single-stranded, telomeric DNA (Harrington et al., 1997).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TEP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu2368-Glu2627 of human TEP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
