Trichohyalin confers mechanical strength to the hair follicle inner root sheath and to other toughened epithelial tissues, such as the hard palate and filiform ridges of the tongue, by forming multiple complex crosslinks with itself and with other structural proteins.
The predicted protein sequence consists of full and partial repeats of a 23aa sequence that could form an alpha-helical rod, followed by a C-terminal domain.
In situ hybridization of sheep wool follicles showed that trichohyalin was expressed in both the inner root sheath and medulla cells.
It was also expressed in the ventral matrix of human nail, with weaker staining of the nail plate, but no staining of the nail bed.
Trichohyalin partially colocalized with filaggrin (FLG) in human tongue epithelium.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TCHH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe1851-Pro1943 of human TCHH, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
