84-4438-53　TBX21 (T-Box Protein 21, TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet) 200ul　365239

特徴

• TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells.
• Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production.
• Retroviral gene transduction of TBX21 into polarized Th2 and Tc2 primary T cells redirected them into Th1 and Tc1 cells, respectively, as evidenced by the simultaneous induction of IFNG and repression of interleukin-4 (IL4) and interleukin-5 (IL5).
• The murine Tbx21 gene encodes a deduced 530aa protein containing a 189aa T-box DNA-binding domain that shows homology to murine Tbr1) and Xenopus eomesodermin (EOMES).
• The authors subsequently cloned human TBX21, which encodes a 535aa protein that shares 88% aa identity with murine Tbx21.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- K QGRRMFPFLS FTVAGLEPTS HYRMFVDVVL VDQHHWRYQS GKWVQCGKAE GSMPGNRLYV HPDSPNTGAH WMRQEVSFGK LKLTNNKGAS NNVTQMIVLQ SLHKYQPRLH IVEVNDGEPE AACSASNTHV FTFQETQFIA VTAYQNAEIT QLKIDNNPFA KGFRENFESM YASVDTSVPS PPGPNCQLLG GDPFSPLLSN QYPVPSRFYP DLPGQPKDMI SQPYWLGTPR EHSYEAEFR
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap