TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells.
Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production.
Retroviral gene transduction of TBX21 into polarized Th2 and Tc2 primary T cells redirected them into Th1 and Tc1 cells, respectively, as evidenced by the simultaneous induction of IFNG and repression of interleukin-4 (IL4) and interleukin-5 (IL5).
The murine Tbx21 gene encodes a deduced 530aa protein containing a 189aa T-box DNA-binding domain that shows homology to murine Tbr1) and Xenopus eomesodermin (EOMES).
The authors subsequently cloned human TBX21, which encodes a 535aa protein that shares 88% aa identity with murine Tbx21.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat TBX21.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu241-Arg493 of rat TBX21, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
