Exposure to vitamin D3 (1,25-dihydroxyvitamin D3) or phorbol ester induces the bipotent HL-60 cell promyelocytic leukemia cell line to differentiate into monocytes/macrophages, whereas retinoic acid and dimethylsulfoxide induce differentiation towards granulocytes.
The differentiation is accompanied by regulation of MYC, FOS, FMS (CSF1R), and myeloblastin (PRTN3).
By differential screening of HL60 cell lines, Chen and DeLuca (1994) identified a cDNA encoding TXNIP, which they termed VDUP1.
The deduced TXNIP protein has 391aa.
Ribonuclease protection analysis showed dramatically increased expression of TXNIP in response to vitamin D3 but not to phorbol ester.
Chen and DeLuca (1994) concluded that TXNIP is not involved in the differentiation process.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse TBP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg30-Glu381 of mouse TBP2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
