By large scale screening of a human 18-week fetal brain cDNA library, Li et al.
(2004) cloned C21ORF7, which they called TAK1L.
The deduced 242aa protein has a calculated molecular mass of 27.3kD and shares homology with the C-terminal domain of TAK1 (MAP3K7; 602614).
Northern blot analysis detected expression of a 2.0-kb TAK1L transcript in human peripheral blood leukocytes but not in 7 other tissues tested.
PCR analysis detected TAK1L expression in all fetal and adult tissues tested with the exception of brain, fetal heart, and fetal skeletal muscle.
PCR analysis of tumor cell lines showed TAK1L expression in breast carcinoma and colon and prostatic adenocarcinoma cell lines.Li et al.
(2004) determined that the TAK1L gene contains 9 exons and spans 80 kb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TAK1l.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ser242 of human TAK1l, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
