By large scale screening of a human 18-week fetal brain cDNA library, Li et al.
(2004) cloned C21ORF7, which they called TAK1L.
The deduced 242aa protein has a calculated molecular mass of 27.3kD and shares homology with the C-terminal domain of TAK1 (MAP3K7; 602614).
Northern blot analysis detected expression of a 2.0-kb TAK1L transcript in human peripheral blood leukocytes but not in 7 other tissues tested.
PCR analysis detected TAK1L expression in all fetal and adult tissues tested with the exception of brain, fetal heart, and fetal skeletal muscle.
PCR analysis of tumor cell lines showed TAK1L expression in breast carcinoma and colon and prostatic adenocarcinoma cell lines.
Li et al.
(2004) determined that the TAK1L gene contains 9 exons and spans 80kb.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended DilutionS:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control Included:365224A: TAK1L, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes mouse TAk1L.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ser142 of mouse TAK1l, expressed in E. coli.
