he synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2 ) sensors in the process of vesicular trafficking and exocytosis.
Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
Synaptotagmin, a highly conserved synaptic vesicle protein, binds calcium at physiologic concentrations in a complex with negatively charged phospholipids.
This binding is specific for calcium and involves the cytoplasmic domain of synaptotagmin.
Calcium binding is dependent on the intact oligomeric structure of synaptotagmin; it is abolished by proteolytic cleavage at a single site.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SYT1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu136-Lys422 of human SYT1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
