STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway.
The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters.
The deduced 547aa protein has a calculated molecular mass of 59.6kD.
STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region.
Northern blot analysis detected ubiquitous expression of a 3.8-kb transcript, with most abundant expression in brain and pancreas.
Western blot analysis of mouse tissues detected a protein of about 60kD in all tissues examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human STK39.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Tyr63-Ser545 of human STK39, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
