STAP1 functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling.
The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity.
A mouse ortholog, stem cell adaptor protein 1, shares 83% identity with its human counterpart.
The BRDG1 open reading frame encodes a protein of 295aa.
The overall structure of BRDG1 resembles those of docking proteins such as IRS1, IRS2, DOK1, or DOC2.
BRDG1 is phosphorylated by TEC directly in vitro.
Studies revealed, furthermore, that BRDG1 appears to function as a docking protein acting downstream of TEC in BCR signaling.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat STAP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Ala297 of rat STAP1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
