84-4437-97　SSRP1 (Structure Specific Recognition Protein 1, FACT, FACT80, T160, Facilitates Chromatin Remodeling 80kD Subunit, Recombination signal sequence recognition protein 1, Chromatin-specific transcription elongation factor 80) 200ul　365182

特徴

• Structure specific recognition protein 1 (SSRP1) is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT.
• FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation.
• FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin.
• When a transcribing RNA polymerase II approaches, FACT promotes disruption of the histone octamer by binding and removing 1 or both histone H2A/H2B dimers.
• This protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA.
• This protein also functions as a co-activator of the transcriptional activator p63.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EFNDV YQEVKGSMND GRLRLSRQGI IFKNSKTGKV DNIQAGELTE GIWRRVALGH GLKLLTKNGH VYKYDGFRES EFEKLSDFFK THYRLELMEK DLCVKGWNWG TVKFGGQLLS FDIGDQPVFE IPLSNVSQCT TGKNEVTLEF HQNDDAEVSL MEVRFYVPPT QEDGVDPVEA FAQNVLSKAD VIQATGDAIC IFRELQCLTP RGRYDIRIYP TFLHLHGKTF DYKIPYTTVL RLFLLP
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.