Sperm surface molecules have been shown to be transferred to the egg membrane during fertilization.
Thus, the sperm cell can share in or impart antigenic specificities to fertilized ova and cleaving embryos.
The molecules that are incorporated into the oocyte may provide an extranuclear signal to the oocyte to cleave.
The sperm surface antigen involved in some step of early cleavage of the fertilized oocyte is a doublet of proteins of approximately 14kD and 18kD.
Antibodies to this protein inhibit early cleavage of the oocyte without affecting pronuclear formation.No homology was found to any known sequence, indicating that CS1 is a unique protein.
In a rabbit reticulocyte in vitro translation system, the transcribed CS1 RNA produced a 33kD CS1 protein.
The 2 parts of the antigen are presumably derived from 1 transcript.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SSFA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Lys587-Pro802 of mouse SSFA2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
