84-4437-87　［受注停止］SRPK2 (SRSF Protein Kinase 2, SFRSK2, SFRS Protein Kinase 2, Serine/arginine-rich protein-specific kinase 2) 200ul　365172

特徴

• Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression.
• This process requires small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP splicing factors, both of which are stored in nuclear 'speckles.' Non-snRNP factors containing an arginine/serine-rich (RS) domain, designated 'SR proteins', are phosphorylated, predominantly on serines within their RS domains.
• The predicted Srpk2 protein contains conserved serine/threonine kinase domains, a proline-rich domain, a basic region which may be a nuclear localization signal, and an acidic domain.
• The mouse Srpk2 protein is 58% and 32% identical to the human SRPK1 and yeast Dsk1 proteins, respectively.
• The authors demonstrated that Srpk2 phosphorylated ASF/SF2 in vitro.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- E LANIDPTWIE SPKTNGHIEN GPFSLEQQLD DEDDDEEDCP NPEEYNLDEP NAESDYTYSS SYEQFNGELP NGRHKIPESQ FPEFSTSLFS GSLEPVACGS VLSEGSPLTE QEESSPSHDR SRTVSASSTG DLPKAKTRAA DLLVNPLDPR NADKIRVKIA DLGNACWVHK HFTEDIQTRQ YRSIEVLIGA GYSTPADIWS TACMAFELAT GDYLFEPHSG EDYSRDEDHI AHIIELL
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.