The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins.
The SRP contains a core RNA subunit with which six proteins are assembled.
Some Alu transcripts are converted by 3-prime processing to a poly(A)-, monomeric species known as small cytoplasmic (sc) Alu RNA.
The 'Alu domain' of SRP consists of 2 proteins referred to as SRP9 (9 kD) and SRP14 and the Alu-homologous region of 7SL RNA.
Information on SRP9 and SRP14 has been obtained from the study of the genes in the dog and mouse, respectively.
The human and mouse SRP14 proteins are about 90% identical (Chang et al., 1994).
Hsu et al.
(1995) cloned the human SRP9 cDNA and showed that the predicted protein was approximately 96% similar to its canine homolog.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SRP9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro2-Glu86 of human SRP9, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
