The 22kD sorcin protein was overexpressed in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic lymphoma cell line.
By PCR with primers based on the sequence of hamster sorcin, the authors recovered a partial human sorcin cDNA.
Using the partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs corresponding to the entire sorcin coding region.
The predicted 198aa human protein shares 95% sequence identity with hamster sorcin
Human sorcin contains 4 EF-hand calcium-binding motifs.
Purified protein bound calcium in vitro.
Southern and Northern blot analysis revealed that the sorcin gene is amplified and overexpressed in HOB1/VCR-1.0 cells.
No expression was detected in parental HOB1 cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.v
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SRI.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly29-Leu169 of human SRI, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
