SPRED2 is a member of the Sprouty/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade.
The deduced 411aa protein contains an N-terminal Enabled (ENA)/VASP homology-1 (EVH1) domain, a central KIT-binding domain (KBD), and a C-terminal SPRY domain.
Endogenous Spred2 localized to the membrane in rat PC12 cells, and mutation analysis determined that the C-terminal SPRY domain was required for plasma membrane localization.Inhibition appeared to occur through the formation of a Spred-Ras complex that inhibited activation of MAP kinase by suppressing phosphorylation and activation of Raf.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SPRED2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Ala418 of human SPRED2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
