84-4437-72　SPON2 (Spondin 2, DIL1, Mindin, M-spondin, Extracellular Matrix Protein, Differentially expressed in cancerous and non-cancerous lung cells 1) 200ul　365157

特徴

• The full-length SPON2 cDNA encodes a 331aa protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1): a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat
• The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins.
• RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues.
• Immunoblot blot analysis showed expression of a 42kD protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VH AVFSAPAIPS GTGQTSTELE VHPRHSLVSF VVRIVPSPDW FVGIDSLDLC EGGRWKEQVV LDLYPHDAGT DSGFTFSSPN FATIPQDTVT EITASSPSHP ANSFYYPRLK SLPPIAKVTF VRLQQSPRAF APPSLDLASR GNEIVDSLSV PETPLDCEVS LWSSWGLCGG PCGKLGAKSR TRYVRVQPAN NGTPCPELEE EAECAPDNCV
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.