SPINT2 encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases.
The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor.
This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea.
Multiple transcript variants encoding different isoforms have been found for this gene.
SPINT2 protein is structurally similar to SPINT1 and contains an N-terminal putative signal peptide sequence, a hydrophobic, membrane associated C-terminal region, and a central region that encodes the processed, cleaved protein.
SPINT2 has 2 potential N-glycosylation sites, but a different N-linked sugar chain from that of SPINT1.
Two regions show extensive similarity to the Kunitz-type sequence of serine protease inhibitors.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SPINT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala28-Lys197 of human SPINT2, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
