The N terminus of SP140, between 29aa and 157aa, is 49% identical to a corresponding N-terminal region of SP100.
The C terminus of SP140 contains a cysteine-rich zinc finger motif and a bromodomain.
SP140 also has a central nuclear localization signal.
RNA dot blot analysis detected high SP140 expression in spleen and peripheral blood leukocytes, with much lower levels in thymus, prostate, ovary, small intestine, and colon; other tissues showed little to no expression.
Low expression of a smaller transcript was also observed.
Western blot analysis with PBC autoantibodies detected SP140 at an apparent molecular mass of 140 kD following in vitro transcription and translation.
Immunohistochemistry demonstrated that SP140 colocalized with PML in dense nuclear bodies in activated myeloid precursor cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Sp140.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly443-Met655 of human Sp140, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
