Sp100 nuclear antigen is an interferon stimulated antigen found in the bile duct of primary biliary cirrhosis.
Histologically sp100 'dots' regions of the cell nucleus.
Viral infection and mitogens affect the expression of the Sp100 autoantigen.
Cells grown in the presence of interferons (alpha, beta, and gamma) revealed an increase both in size and number of the Sp100 protein-containing nuclear dots and increase the protein concentration.
This raises "the question whether cytokine-mediated increase of Sp100 protein expression plays a role in induction of anti-Sp100 autoantibodies."Some sp100 variants contain a domain similar to two interferon-inducible nuclear phosphoproteins, suppressin and DEAF1.
This defines a novel protein motif, the HNPP-box.
Another class of variants has high mobility group 1 (HMG1) protein sequence as a domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Sp100.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly610-Lys802 of human Sp100, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
