SOCS-3 is an SH2-containing protein that binds to the activation loop of Janus kinases, inhibiting kinase activity and thereby suppressing cytokine signaling.
It is a member of the STAT-induced STAT inhibitor (SSI).
To date, there are eight members of SOCS family that have been recognized.
Structurally, the SOCS proteins are composed of an N terminal region of variable length and amino acid composition, a central SH2 domain and a previously unrecognized C-terminal motif that has been called the SOCS box.
The expression of the SOCS-3 gene is induced by various cytokines, including IL-6, IL-10, and interferon (IFN)-gamma.
Studies of the mouse counterpart of the SOCS-3 gene suggested this gene is involved in the negative regulation of fetal liver hematopoiesis, and placental development.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SOCS3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Leu225 of human SOCS3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
