SMAD5 or Mothers against decapentaplegic homolog 5 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decapentaplegic".
It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators.
Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's).
The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein.
SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Smad5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly210-Val464 of human Smad5, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
