Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion.
Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A6, permit cellular efflux of zinc.
The deduced mouse and human proteins contain 460aa and share 92% identity.
Mouse Znt6 has 6 transmembrane domains, a loop between transmembrane domains 4 and 5, and cytoplasmic N and C termini.
In contrast to other Znt proteins, the loop region of Znt6 lacks multiple histidines.
Normal rat kidney cells expressed Znt6 in a perinuclear location and in a punctate/tubular pattern throughout the cytoplasm and under the plasma membrane.
Disruption of the Golgi compartment also disrupted Znt6 localization.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SLC30A6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val253-Pro458 of mouse SLC30A6, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
