84-4436-95　SLC30A5 (Solute Carrier Family 30, Member 5, ZTL1, ZnT-5, ZNT5, ZNTL1, Zinc Transporter 5, ZnT-like transporter 1) 200ul　365081

特徴

• The deduced 523aa protein contains 12 predicted membrane-spanning domains with extracellular N and C termini.
• Between transmembrane domains 9 and 10, ZTL1 has a zinc-binding histidine-rich intracellular loop.
• ZTL1 shares 34% identity with ZNT1 (SLC30A1) over a 122aa stretch, and it shares significant homology with several rodent zinc transporters.
• Northern blot analysis detected 2.0- and 5.0-kb transcripts in CaCo2 cell line RNA.
• RT-PCR of mouse tissues detected ubiquitous expression, with highest level in kidney and lowest level in liver.
• The deduced 765aa protein has 15 predicted membrane-spanning domains and a calculated molecular mass of about 84kD.
• Western blot analysis of the full-length protein expressed in HeLa cells indicated an apparent molecular mass of about 55kD.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control Included:365081A: SLC30A5, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
• DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
• Stable for 6 months after receipt.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.