By screening a human temporal cortex cDNA library with a mouse zinc transporter-3 (Znt3) cDNA, Palmiter et al.
(1996) cloned cDNAs encoding ZNT3.
The predicted 388aa ZNT3 protein has 6 transmembrane domains, with the N and C termini located in the cytoplasm.
The human and mouse ZNT3 proteins are 86% identical.
Northern blot and RT-PCR analyses of mouse tissues detected Znt3 transcripts only in brain and testis.
By in situ hybridization of mouse brain, Znt3 mRNA was most abundant in the hippocampus and cerebral cortex.
Immunohistochemical analysis of brain using antibodies against Znt3 detected protein expression in a pattern similar to that of histochemically localized vesicular zinc.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SLC30A3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg286-Ala388 of human SLC30A3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
