CD84 encodes a 328aa protein that is shortened to 307aa after cleavage of the signal peptide.
The protein has 4 potential N-linked and 29 potential O-linked glycosylation sites.
The observed molecular mass on SDS-PAGE is 64 to 82kD, which endoglycosidase treatment reduces to 46kD and 36kD products, close to the calculated mass of approximately 35kD.
In addition to a 25aa transmembrane region and an 83aa cytoplasmic region, CD84 has a 189aa extracellular segment composed of 2 Ig-like domains similar to that found in other members of the CD2 family.
Northern blot analysis revealed mRNA expression of a 4.4-kb transcript at high levels in lymphoid tissue and at lower levels in lung; no expression was found in liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SLAMF5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile32-Leu222 of mouse SLAMF5, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
