SIAL2 belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids.
Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase.
Its cytosolic localization was demonstrated by cell fractionation experiments.
The 2 putative exons of NEU2 encode a deduced 380aa protein with a calculated molecular mass of 42.23kD.
The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases.
It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1.
NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat SIAL2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to His185-Gln379 of rat SIAL2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
