Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates.
In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60 % aa identity.
Human Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region
The mature region is autocatalytically processed into a nonglycosylated, 20kD, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34kD, 239 aa C-terminal fragment.
The 20kD Shh-N fragment is the core of the active hedgehog molecule.
It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat SHH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ser237-Arg399 of rat SHH, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
