Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates.
In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60aa identity.
Human Shh is synthesized as a 437aa precursor that contains a 24aa signal sequence and a 413aa mature region.
The mature region is autocatalytically processed into a nonglycosylated, 20kD, 174aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34kD, 239aa C-terminal fragment.
The 20kD Shh-N fragment is the core of the active hedgehog molecule.
It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SHH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro36-Ala193 of human SHH, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
